angelica dahurica meaning in Chinese
大活
独活
或杭白芷
Examples
- Studies on cultivation measures of angelica dahurica
白芷栽培措施研究 - Chemical constituents of angelica dahurica
中药川白芷化学成分的研究 - Objective to study the accumulation of dry matter and changes of isoimperatorin content and accumulation in the root of angelica dahurica in different growth and development duration
摘要目的明确白芷不同生长时期干物质积累及根中异欧前胡素质量分数和积累量变化。 - In the process of study for the extracellular cam , the research in our lab indicates that cambps also possibly exist in cell wall area . for the first time , an extracellular ca ~ ( 2 + ) - dependent cambp ( named : ecbp21 ) was purified from 0 . 1mol / l cacl _ ( 2 ) extracts of angelica dahurica suspension - cultured cells ( tang et al . 1996 )
我室在对植物细胞外cam的研究过程中，发现细胞外还可能存在cambps ，并首次从白芷悬浮培养细胞外纯化了一种依赖ca ~ ( 2 + )的、分子量约为21kd的细胞外cambp ( tang等， 1996 ) (定名为： ecbp21 ) 。 - Then using ecbp21 antibody and immunogold transmission electron microscopy method , we studied the subcellular localization of ecbp21 . the results indicated that the gold particles were mainly localized in the cell wall in callus cells and rachis cells of angelica dahurica . these results indicated that ecbp21 mainly localized in cell wall , which provide a direct evidence of the extracellular existence of ecbp21 . furthermore , using ecbp21 antibody and immunohistochemical method , we studied the organic specially distribution of ecbp21 , the results indicated that ecbp21 distributed in all organize , but it distributed more in leave n flower rachis than in leafstalk and root
首先，构建了ecbp21表达载体，诱导了重组蛋白的表达，并通过胶回收法获得了大量纯化重组ecbp21蛋白，制备了高效价、高特异性抗体；随后，利用ecbp21抗体，结合免疫胶体金电镜定位技术进行了ecbp21亚细胞定位研究，结果显示：在白芷愈伤组织细胞和花序轴细胞中金颗粒主要分布在细胞壁区域，而在细胞内未发现或仅有少量金颗粒分布，表明ecbp21蛋白主要定位于细胞壁区域，这为细胞外cambp ( ecbp21 )的胞外存在提供了直接证据：进一步，利用ecbp21抗体，通过免疫组织化学分析研究了ecbp21组织特异性分布状况，结果表明ecbp21在白芷各组织中均有分布，但在叶、花、花序轴中分布较多，而在叶柄、根中分布较少。